Funsho Ogunshola, SANTHE PhD trainee based at the Africa Health Research Institute (AHRI), recently spent four weeks at Professor John Wherry’s laboratory at the Perelman School of Medicine, University of Pennsylvania, USA, learning how to conduct an ATAC-Seq assay and how to analyse sequencing data generated using bio-informatics tools.
The Assay for Transposase Accessible Chromatin using Sequencing (ATAC-Seq), is a new technique used to investigate the epigenetic state of different lineages of immune cells and is crucial to Ogunshola’s PhD project to assess the epigenetic state of T cells in the lymphoid tissues of HIV infected subjects.
“This new skill will be important for the completion of my PhD study and will also benefit other researchers at our institute who are interested in using this assay to answer their research questions,” says Ogunshola. “I had a great time in John’s Lab and I’m grateful to SANTHE for giving me such a valuable opportunity to learn this unique technique. This was truly a remarkable experience for me and I am looking forward to setting up the assay in our own laboratory.”