Kenya has recently launched Pre-Exposure prophylaxis (PrEP) for individuals at high risk of HIV acquisition following strong recommendations by WHO in 2015. Mwashigadi’s research project seeks to explore the barriers and facilitators to PrEP uptake and adherence among men who have sex with men at Mtwapa, Kenya. The study uses qualitative approach to review the existing uptake of PrEP in the high-risk cohort of MSM, and identify participants who either started and stopped taking PrEP, who are eligible for PrEP but did not want to take PrEP, had very poor adherence based on blood Tenofovir levels measured or those initiated on PrEP and have maintained good adherence over time. The study population are drawn from the ongoing prospective open cohort and observational feasibility study to assess recruitment and retention and estimate HIV incidence for an HIV vaccine efficacy trial. As PrEP delivery continue to roll out nationally in Kenya and other African countries, these findings will help inform strategies to improve PrEP uptake and adherence among key populations who are at substantial risk of HIV acquisition.